• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A heme concentrate containing 15 to 55 percent heme in readily absorbable form and useful as a pharmaceutical against anemia and as an iron-enriching agent in foodstuffs, and a method for the preparation of said heme concentrate of a mixture of heme and blood substance obtained when cleaving hemoglobin, for example, from whole blood or red blood cells, by treating said mixture with a dehydrating agent, preferably a lower alcohol or a mixture of alcohols, either at a pH of at least 8.0 or in the presence of a substance promoting the separation, preferably imidazole or an imidazole derivative, at a pH of at least 6.0, preferably 6.5 to 8.5, whereafter the solid blood substance is separated and the heme concentrate is recovered from the remaining solution; the blood substance simultaneously recovered, mainly globin, is intended for purposes of foodstuffs and fodder.
    公开号:SU1660573A3
    申请号:SU813359217
    申请日:1981-11-30
    公开日:1991-06-30
    发明作者:Геран Сигвард Линдроос Пол
    申请人:Пол Геран Сигвард Линдроос (SE)；
    IPC主号:
    专利说明:

    The invention relates to medicine, namely to the medical industry and concerns a method for producing a concentrate.
    The purpose of the invention is to increase the concentration of the topic in the concentrate.
    According to this method, a concentrate is extracted from a theme with blood substance obtained by splitting hemoglobin, and the concentrate is separated from globin in such a way that the heme concentrate turns into a dissolved form, while the rest of the substance is in undissolved form .
    EXAMPLE 1 Blood drawn from the body with the addition of sodium citrate as a coagulant is separated by centrifugation, resulting in a plasma and a solution of red blood cells. Sodium chloride and ethanol are added to a liquid solution of red blood cells in such an amount that 2.2 g of a mixture of red blood cells containing 0.34 g of protein, 0.16 g of sodium chloride, 0.26 g of 100% - are obtained. pure ethanol, 1.44 g of water and soluble substances present in the blood. Protein, mainly hemoglobin, is determined by precipitation with ethanol of 2.2 g of a mixture of blood cells containing 11.1 mg of heminum, as determined by the method of pyridine.
    A mixture of 4 ml of 96% (by volume) ethanol and 0.8 ml of 1 M HCI at a temperature of –8 ° C is added dropwise with simultaneous stirring and cooling in 2.2 g of a mixture of red blood cells at -5 ° s After this addition, the pH is 3.1 and the temperature is -5 ° C. 3 ml of ice water and 1.8 ml of 0.5 M NaOH in 70% (by volume) ethanol are added to the mixture while stirring and cooling; the temperature of the mixture is -5 ° C, and the value
    § cl
    XI SA)
    Igj
    The pH is 7.5. After 5 minutes, 10 ml of 96% (by volume) ethanol, 10 ml of 70% (by volume) ethanol, 1 ml of 1 M glycine in aqueous solution and 2.2 ml of 0.5 M NaOH were added to the mixture. , while stirring and cooling. The mixture then contains 67% (by volume) ethanol, the pH is 10.3, and its temperature is -5 ° C. The mixture is centrifuged at 3000 g for 5 minutes, resulting in a floated layer of red and a brownish-red paste. The supernatant contains 0.18 mg heme / ml (61.5% of the content of the topic of its content in the starting material),
    This paste is processed for the third time as described above, with the result that a theme content of 0.06 mg / ml is reached (5.4% theme content of the content in the starting material). The overall concentrate yield is 78.6%. This paste is brown in color and its dry weight is 0.27 g (protein yield 79.5%).
    PRI mme R 2. Having carried out the process in the same manner as in Example 1, the pH was lowered to 3.1, and then the pH was adjusted to 7.5. The mixture was extracted in the same manner as in Example 1, but entering simultaneously 1 ml of 2 M imidazole in 70% ethanol (by volume). The pH is adjusted to 8.4. Repeat the operation two more times. The layers that float to the surface contain, respectively, 79, 12 and 4% of the topic of its content in the starting material. The overall concentrate yield is 95%. This paste has a light brown color and contains 85% of the protein content of the starting material.
    Para and p 3. A mixture of 2 ml of water and 0.7 ml of hydrochloric acid (1 M) at 1 ° C is introduced dropwise while stirring and cooling in 2.2 g of a mixture of red blood cells at - 8 ° C. After that, the pH was adjusted to 3.2 and the temperature was adjusted to -5 ° C. Then 4 ml of 96% (by volume) ethanol, 10 ml of 70% (by volume) ethanol, 1 ml of 1 M glycine in the form of an aqueous solution, 1 ml of 2 molar imidazole in 70% - are added into this mixture. nom (by volume) ethanol and 1.5 ml of 0.5 molar NaOH in 70% (by volume) ethanol, pH 8.0 while stirring and cooling; the temperature of the mixture is maintained at -5 ° C, the ethanol content is about 56% by volume. The mixture is centrifuged after 5 minutes, as in example 1, and get-floated layer containing 59% theme from the source material, and a brown paste, Paste extracted twice
    10 mm of 70% (by volume) ethanol each time at a pH of 8.0 and floating layers are obtained, containing respectively 18 and 8% of the content of the starting material, the total concentrate yield is 85%. The paste is brown and has a protein content of 72% of the content in the starting material.
    EXAMPLE 4 A mixture of 3 ml of 96% (by volume by volume) ethanol and 0.35 ml of 0.5 M NaOH in 70% (by volume) ethanol at -10 ° C is added dropwise. m while stirring and cooling in 2.2 g of a mixture of blood cells at -10 ° C. After this, the pH value is 10.2 and the temperature is -10 ° C. To this solution is added a mixture of 2 ml of ice water, 3 ml of 50% (by volume) ethanol and 0.15 ml of 1 M HCI at -10 ° C. The pH of the mixture is 0 liter 7.5 and the temperature is -10 ° C. A mixture of 5 ml of 96% (by volume) ethanol, 5 ml of 70% (by volume) ethanol and 2 ml of 0.5 M NaOH in 70% (by volume) ethanol is added to this solution at -10 ° s The pH value of 5 of the resulting mixture is 10.3, the temperature is -10 ° C, the ethanol content is about 64% (by volume). After 5 minutes, the mixture was centrifuged, as in Example 1, to give a supernatant layer containing 0% 46% of the content in the starting material and a dark brown paste. The paste is extracted twice with 10 ml of 65% (by volume) ethanol, each time at a pH of 10.3 and at -10 ° C, and 5 layers emerge, containing respectively 14 and 7% of the content of source material. The overall concentrate yield is 67%. The resulting paste is brown and has a protein content of 71% of the 0 content in the starting material.
    PRI me R 5. Having carried out the process in the same manner as in Example 4, the pH of the mixture of blood cells was raised to 10.2 and the temperature was maintained at 0 ° C. In 5, this solution is injected with a mixture of 3 ml of 50% (by volume) ethanol and 0.2 ml of 1 M HCI at 0 ° C. The pH of the mixture is 8.2 and the temperature is 0 ° C. Then a mixture of 5 ml of 96% (by volume) ethanol and 10 ml of 70% (by volume) ethanol is introduced into this solution at 0 ° C. The pH of the resulting mixture is 8.2, the temperature is 0 ° C and the ethanol content is 66% (by volume). After 5 minutes, the mixture is centrifuged, as in Example 1. A supernatant layer containing 52% of the content of the raw material and a dark brown paste are obtained. The paste is extracted twice with 10 ml of 65% (by volume) ethanol and 0.2 ml of imidazole in 70% (by
    volume) ethanol, each time at a pH of 8.2 and at 0 ° C, and the resulting layers with a content of 28% and 12%, respectively, of its content in the starting material. The overall concentrate yield is 92%. The resulting paste is brown and contains 85% protein from the source material.
    EXAMPLE 6 A mixture, 1 ml of water and 0.8 ml of 0.5 M NaOH at 10 ° C are added dropwise while stirring in 2.2 g of a mixture of blood cells at a temperature of 10 ° C. After this, the pH is 11.0 and the temperature is 10 ° C. 4 ml of 96% (by volume) ethanol, 1 ml of 2 M imidazole in 70% (by volume) ethanol, 1 ml of 30% sodium citrate in the form of an aqueous solution and 0.5 ml are introduced into the resulting solution. 1 M HCI. The pH of the mixture obtained is 7.5 and the temperature is 10 ° C. After this, 3 ml of 96% (by volume) ethanol and 10 ml of 70% (by volume) ethanol are introduced into the mixture. The pH of the mixture is 7.5, the temperature is 10 ° C, and the ethanol content is about 64% (by volume). After 5 minutes, the mixture was centrifuged, as in Example 1, and a floating layer containing 53% of the content of the starting material and a dark brown paste were obtained. The paste is extracted twice with 10 ml of 65% (by volume) ethanol and 0.1 ml of 2 M imidazole in 70% (by volume) ethanol, each time at pH 7.5 and at 10 ° C, and the result is emerging layers, containing respectively 26 and 13% of the topic of its content in the source material. The overall concentrate yield is 92%. The paste has a brown color and contains 74% of its protein content in the starting material.
    Example. A mixture of 5.5 ml of 96% (by volume) ethanol and 1.0 ml of 1-molar HCI at a temperature of -18 ° C is added dropwise with simultaneous stirring and cooling into a mixture of 2.2 g of a mixture of blood glue - current, 0.1 ml of 1 M glycine in the form of an aqueous solution, 0.1 ml of 2 M imidazole in 70% (by volume) ethanol and 2 ml of 50% (by volume) ethanol at -18 ° C . Thereafter, the total / gyoshina pH is 3.0 and the temperature is -18 ° C. Into the resulting solution, 3 ml of 40% ammonium sulfate are introduced in the form of an aqueous solution while stirring and cooling. After 5 minutes, a mixture of 10 ml of 96% (by volume) ethanol, 20 ml (by volume) of ethanol and 4 ml of 0.5 M NaOH in 70% (by volume) ethanol at -18 ° was introduced into the solution. C, while stirring and cooling. Thereafter, the pH value is 7.5 and the temperature is -18 ° C, the ethanol content is about 69% (by volume). The mixture was centrifuged as in Example 1. The resulting paste was extracted twice with 20 ml of 70% (by volume) ethanol, 0.1 ml of 1 M glycine as an aqueous solution and 0.1 ml of 2 M imidazole in 70% (by volume) ethanol each time at pH 7.5 and at -18 ° C. The content of the topic in the pop-up layer is respectively 69.14 and 5% of its content in the starting material. The overall concentrate yield is 88%. The resulting paste has a light brown color and has a protein content of 81% of its content in the starting material.
    Example A mixture of 8 ml of 96% (by volume) ethanol and 0.8 ml of 1 M HCI at -5 ° C is added dropwise while stirring and cooling in 2.2 g of a mixture of red blood cells at -5 ° WITH. After this, the pH is 3.1 and the temperature is -5 ° C. 0.8 ml of 0.2 M NaOH in 50% (by volume) ethanol and 10 ml of 50% (by volume) ethanol are added to this mixture, after which the pH of the mixture is 3.5 and the temperature is -5 ° s The mixture was centrifuged at 9000 g for 5 minutes, after which a yellow supernatant and a black paste were obtained. A gray protein containing 0.21 g of dry matter precipitates from the supernatant after adding 8 ml of water to it at pH 7.5. Heme paste of black color is mixed with a mixture of 30 ml of 70% (by volume) ethanol, 0.5 ml of 1 M glycine in the form of an aqueous solution, 0.5 ml of 2 M imidazole in 70% (by volume) ethanol and 2 , 5 ml of 0.5 M NaOH in 70% (by volume) ethanol. The pH of the mixture is 8.6, the temperature is -5 ° C, and the ethanol content is about 70% (by volume). The mixture is centrifuged at 3000 g for 5 minutes and a supernatant layer containing 72% of its heme content in the starting material and a brown paste are obtained. The paste is extracted twice with 30 ml of 70% (by volume) ethanol and 0.1 ml of 2 M imidazole in 70% (by volume) ethanol, each time with a pH of 8.6 and at -5 ° C to obtain heme layers with a heme content of 19 and 8% of its content in the starting material, respectively. The overall yield of heme concentrate is 99%. The resulting paste has a greenish-brown color and contains 0.1 g dry weight. The total protein yield is 91%.
    PRI me R 9. a. Carrying out the process as in Example 8, black heme paste is separated when using 2.2 g of a mixture of blood cells as starting material. The paste is mixed with 30 ml of 50% (by volume) ethanol and pH
    adjusted to 8.5 with 0.5 M NaOH in 70% (by volume) ethanol at -1 ° C. The ethanol content is about 50% (by volume). The mixture was centrifuged as in Example 8 and a supernatant was obtained, in which the content of the topic was 33% of its content in the starting material, and a dark brown paste. This paste is extracted three times with 30 ml of 50% (by volume) ethanol, each time at a pH of 9.0 and at -1 ° C, and floating layers are obtained with a content of 24, 13 and 9%, respectively, of its content in the original material. The overall concentrate yield is 79%. This paste is brown in color and has a dry mass content of 0.1 g. The total protein yield is 91%.
    b. Carrying out the process as in Example 8, the separation of black gem paste takes place when using 2.2 g of a mixture of blood cells as the starting material. The paste is mixed with 30 ml of 40% (by volume) ethanol and the pH is adjusted to 10.7 with 0.5 M NaOH in 70% (by volume) ethanol. The temperature is -4 ° C and the ethanol content is about 40% (by volume). The mixture was centrifuged as in Example 8, and a supernatant containing the theme content of 71% of its content in the starting material and a brown paste were obtained. This paste is extracted twice with 30 ml of 40% (by volume) ethanol, each time at a pH of 10.7 and at -4 ° C, and floated layers with a content of 7.2 and 4.8%, respectively, are obtained. its in the source material. The overall concentrate yield is 83%. The resulting paste has a light brown color and contains 0.08 g dry weight. The total protein yield is 85%.
    An example. Carrying out the process as in Example 8, black heme paste was isolated using 2.2 g of a mixture of blood cells as starting material. This paste is stirred with a mixture of 20 ml of 60% (by volume) ethanol, 0.2 ml of 2 M imidazole in 70% (by volume) ethanol and 1 ml of 40% (by weight) ammonium sulfate in the form of an aqueous solution . The pH is adjusted to 6.8 with 0.5 M NaOH in 70% (by volume) ethanol. The temperature is 25 ° C, and the ethanol content is about 60% (by volume). The mixture was centrifuged as in Example 8, and a supernatant with a theme content of 63% of its content in the starting material and a brownish-red paste were obtained. The paste is extracted twice with 60% (by volume) ethanol, imidazole and ammonium sulfate in the indicated amounts, each time with a pH value of 6.8
    and at a temperature of 25 ° C, and floated layers are obtained with a theme content of 14 and 8%, respectively, of its content in the starting material. The overall concentrate yield is 85%. The resulting paste is brown and has a dry weight of 0.07 g. The total protein yield is 83%.
    PRI me R 11. Carried out the process so
    0 as described in Example 8, black heme paste is isolated using 2.2 g of a mixture of blood cells as starting material. This paste is mixed with a mixture of 30 ml of 96% (by volume) ethanol and 0.2 ml of 1 M
    5 glycine in the form of an aqueous solution. The pH was adjusted to 10.4 with 0.5 M NaOH in 70% (by volume) ethanol. The temperature is -20 ° C. ethanol content of about 90% (by volume). The mixture was centrifuged as in Example 8, and a supernatant layer containing 61% of its content in the starting material and a dark brown paste were obtained. The paste is extracted twice with 90% (by volume) ethanol
    5 and glycine in the indicated amounts, each time at a pH of 10.4 and at -20 ° C, floating layers with a theme content of 20 and 9% of its content in the starting material are obtained. Overall output
    0 concentrate subject is 90%. The resulting paste has a light brown color and its dry mass content is 0.1 g. The total protein yield is 91%.
    Example12. Carried out the process so
    5 as in Example 8, black heme paste was isolated using 2.2 g of a mixture of blood cells as starting material. This paste is mixed with a mixture of 30 ml of 40% (by volume) ethanol and 0.2 ml of 2 M imidazole.
    0 in 70% (by volume) ethanol. The VelNur is adjusted to 7.0 with 0.5 M NaOH in 70% (by volume) ethanol. The temperature is -5 ° C and the ethanol content in the mixture is about 42% (by volume). This
    5, the mixture is centrifuged, as in Example 8, and a supernatant containing a theme content of 58% of its content in the starting material and a dark red paste are obtained. This paste is extracted twice with 70% (by volume) ethanol and imidazole in the indicated amounts, each time at pH 7 and at -5 ° C, and floating layers are obtained, containing respectively 26 and 5% of the content of the initial content. material. The 065 concentrate concentrate output is 89%. The resulting paste has a light brown color and its dry mass content is 0.1 g. The total protein yield is 91%.
    EXAMPLE 13: Having carried out the process as in Example 8, black heme is isolated.
    paste, using 2.2 g of a mixture of blood cells as the starting material. This paste is mixed with a mixture of 20 ml of 70% (by volume) methanol and 0.2 ml of 2 M imidazole in 70% (by volume) ethanol. The pH was adjusted to 8.5 with 0.5 M NaOH in 70% (by volume) ethanol. The temperature is -5 ° C, the methanol content is about 60% (by volume). The mixture was centrifuged as in Example 8, and a supernatant containing 67% of the content of the starting material and a brownish-red paste were obtained. The paste is extracted twice with 70% (by volume) ethanol and imidazole in the indicated amounts each time at pH 8.5 and at -5 ° C and floated layers with a content of 18% and 7%, respectively, of its content in the starting material. . The overall concentrate yield is 92%. The resulting paste has a light brown color and contains 0.1 g dry weight. The total protein yield is 91%.
    Example 14: The process was carried out as in Example 8 and black heme paste was isolated using 2.2 g of a mixture of blood cells as the starting material. This paste was mixed with a mixture of 20 ml of 70% (according to volume) propanol and 0.2 ml of 2 M imidazole in 70% (by volume) ethanol. The pH was adjusted to 8.0 with 0.5 M NaOH in 70% (by volume) ethanol. The temperature is -5 ° C, the content of propanol is about 60% (by volume). The mixture was centrifuged as described in example 8, and a supernatant layer containing 66% of the content of the starting material and a brownish-red paste were obtained. The paste is extracted twice with 70% (by volume) propanol and imidazole in the indicated amounts each time at pH 8.0 and at -5 ° C. Floated layers with a content of 16% and 9%, respectively, are obtained. material. The overall concentrate yield is 91%. The resulting paste has a light brown color and its dry mass content is 0.1 g. The total protein yield is 91%.
    Example 15: Performing the process as described in Example 8, black heme paste was isolated using 2.2 g of a mixture of blood cells as the starting material. This paste is mixed with a mixture of 20 ml of 70% (by volume) isopropanol, 0.2 ml of 1 M glycine and 0.2 ml of 2 M imidazole in 70% ethanol (by volume). The pH of the mixture was adjusted to 7.5 with 0.5 M NaOH in 70% (by volume) ethanol. The temperature is -10 ° C, the isopropanol content is about 60% (by volume). The mixture was centrifuged as in Example 8, and a supernatant layer with a theme content of 69% of its content in the starting material 5 and a brownish-red paste were obtained. The paste is extracted twice with 70% (by volume) isopropanol glycine and imidazole in the indicated amounts each time at a pH of 7.5 and at a temperature of 0 -10 ° C and floated layers with a content of 17% and 9% respectively are obtained. in the source material. The overall concentrate yield is 95%. The resulting paste has a light brown color and its dry weight is 0.1 g. The total protein yield is 91%.
    Example1b. A mixture of 6 ml of 96% (by volume) ethanol and 1 ml of 1 M HCI at -8 ° C is added dropwise while stirring and cooling in 2.7 g of blood at 0.5 ° C. After that, the pH is 3.0 and the temperature is -5 ° C. Then, 2 ml of 40% ammonium sulfate in the form of an aqueous solution and a mixture of 20 ml of 70% 5% (by volume) ethanol, 0.2 ml of 1 M glycine in the form of an aqueous solution and 0.2 ml of 2 M are introduced into this mixture. imidazole in 70% (by volume) ethanol at -8 ° C with simultaneous stirring and cooling. The pH is adjusted to 8.0 by 0 with 0.5 M NaOH in 70% (by
    volume) ethanol. Temperature is
    -8 ° C and ethanol content of about 65%
    (by volume). The mixture is centrifuged as
    in example 8, and get the emerged layer
    5 containing 66% of the topic of its content in the starting material and brownish-red paste. This paste is extracted twice with 70% (by volume) ethanol, ammonium sulfate and imidazole in the indicated amounts, each time at a pH of 8.0 and at -8 ° C, and floated layers with a content of 16 and 7%, respectively, are obtained its content in the source material. The overall yield of the concentrate is 89%.
    5 This paste after washing it with 50% (by volume) ethanol has a grayish-brown color and the total protein yield is 85%.
    PRI me R 17. Using as a starting material 22 g of a mixture of blood
    0 cells, heme paste is isolated, as a result of extraction with a pH of 4.4.4, at -5 ° C and using ethanol with 70% (by volume), the first extraction solution is obtained in an amount of 199 ml s
    5 heme concentration of 0.38 mg / ml, which corresponds to the content of the theme 68 1% of its content in the starting material, the second extraction solution in the amount of 121 ml with a concentration of heme 0 17 mg / ml, which corresponds to the content of 18.6% of
    its content in the starting material, and the third extraction solution in the amount of 118 ml with a theme concentration of 0.08 mg / ml, which corresponds to the content of the theme 8.5% of its content in the starting material. The extraction solutions were combined and acidified to pH 5.1 using 87 ml of 0.1 M HCI at -5 ° C. The resulting black flocculent precipitate is separated by centrifugation at 3,000 d, the weight amount of this precipitate is 4.5 g. This paste is washed three times in 15 ml of ice-water (in each wash), suspended in 15 ml of ice-water The pH was adjusted to 8.2 with 3 ml of 0.2 M NaOH, frozen and dried at a temperature below 0 ° C. The result is 265 mg of black powder with a theme content of 36%. The overall concentrate yield is 86%.
    The results of the analysis of the product,%. C 46.17; H 5.30; CI 10.53; P 2.8; Fe 3.57; N 9.6; K 3.5 and Na 4.1.
    Example 18. As a result of the extraction of heme paste, isolated from a 50 g mixture of blood cells, at a pH value of 8.2, at a temperature of + 1 ° C and using 60% (by volume) ethanol and 4 ml of 2 M imidazole in 70 % (by volume) ethanol receive the first extraction solution in an amount of 494 ml with a concentration of 0.32 mg / ml theme, which corresponds to the content of the theme 62.8% of its content in the starting material, the second extraction solution in the amount of 467 ml with concentration the topic is 0.11 mg / ml, which corresponds to the content of the topic 20.4% of its content in the starting material, and the third ex traction solution in an amount of 479 ml th topic concentration of 0.06 mg / ml, which corresponds to the topic NIJ containing 11.4% of its content in the starting material. The extraction solutions were combined and acidified to pH 4.0 using 240 ml of 0.1 M HCI at 1 ° C. The precipitate formed is separated, as stated above, the amount of this precipitate
    -
    is 8.5 g. The paste is washed three times in ice water, 25 ml each time, suspended in 25 ml of ice water, the pH is adjusted to 8.5 and the suspension is frozen and dried at a temperature below 0 ° C . The result is 510 mg of a black powdered product with a theme content of 42%. The overall concentrate yield is 85%.
    10 Example 19. Carrying out the process as in Example 18, one extraction solution is obtained. The latter is separately acidified to pH 4.0 and the resulting black precipitate is separated, washed and dried 15 at a temperature below 0 ° C as described in Example 18. The concentrate thus obtained contains 55% of the topic.
    权利要求:
    Claims (1)
    [1]
    According to the proposed method, a heme concentrate containing not less than 40% by weight of heme is obtained. This heme concentrate is intended to be used as a pharmaceutical agent against anemia, as well as an iron-enriched agent in food. The product obtained by a known method, contains about 10 wt.% Heme. The invention of the method of obtaining the heme concentrate by means of the red blood cell hemoglobin cleavage in the presence of a dehydrating agent and the separation of heme and globin, that is, and that. that, in order to increase the concentration of heme in the target product, helen paste containing globin is additionally used, in this case, after splitting the pH is first adjusted to 7.5-8.0, and then the target product is extracted with ethanol or methanol, or propanol, or 40 with a mixture of propanol and isopropanol in equal ratios at a concentration of 40-90 vol.% At pH 8.0-10.7 or with the addition of imidazole at pH 6.5-8.5 at temperature {0) - (- 20) ° C and in the presence of chloride salts,
    45 citrate or sodium sulphate or ammonium.
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    同族专利:
    公开号 | 公开日
    EP0056025A1|1982-07-21|
    WO1981002834A1|1981-10-15|
    JPH0134204B2|1989-07-18|
    IN152639B|1984-02-25|
    SE440596B|1985-08-12|
    DK154266C|1989-03-28|
    IE810644L|1981-10-03|
    DK154266B|1988-10-31|
    DK530181A|1981-11-30|
    EP0056025B1|1985-10-09|
    JPS57500431A|1982-03-11|
    SE8002591L|1981-10-04|
    US4431581A|1984-02-14|
    IE51040B1|1986-09-17|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    SE8002591A|SE440596B|1980-04-03|1980-04-03|PROCEDURE FOR PREPARING A HOME CONCENTRATE FROM A MIXTURE OF HOME AND BLOOD SUBSTANCE RECOVERY BY DIVISION OF HEMOGLOBIN|
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